Intravital Imaging of the Human Cornea Reveals the Differential Effects of Season on Innate and Adaptive Immune Cell Morphodynamics

M Wu; X Zhang; S Karunaratne; JH Lee; ER Lampugnani; KJ Selva; AW Chung; SN Mueller; HR Chinnery; LE Downie

Journal article

Intravital Imaging of the Human Cornea Reveals the Differential Effects of Season on Innate and Adaptive Immune Cell Morphodynamics

M Wu, X Zhang, S Karunaratne, JH Lee, ER Lampugnani, KJ Selva, AW Chung, SN Mueller, HR Chinnery, LE Downie

Ophthalmology | Published : 2024

DOI: 10.1016/j.ophtha.2024.04.020

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Abstract

Purpose: Defining how the in vivo immune status of peripheral tissues is shaped by the external environment has remained a technical challenge. We recently developed Functional in vivo confocal microscopy (Fun-IVCM) for dynamic, longitudinal imaging of corneal immune cells in living humans. This study investigated the effect of seasonal-driven environmental factors on the morphodynamic features of human corneal immune cell subsets. Design: Longitudinal, observational clinical study. Participants: Sixteen healthy participants (aged 18–40 years) attended 2 visits in distinct seasons in Melbourne, Australia (Visit 1, November–December 2021 [spring–summer]; Visit 2, April–June 2022 [autumn–winte..

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University of Melbourne Researchers

Mengliang Wu Author

Senuri Karunaratne Author

Anna Lee Author

Edwin R. Lampugnani Author

Related Projects (1)

Redefining the immune landscape of the human ocular surface

Grants

Awarded by Department of Health, State Government of Victoria

Funding Acknowledgements

Victorian Department of Health (Thunderstorm Asthma Research Contract); Leadership orfiduciary rolee AirHealth Pty Ltd.National Health and Medical Research CouncilUnrelated to this research - Azura Ophthalmics, Alcon Laboratories, Cooper Vision Pty Ltd., Novartis Pharmaceuticals, Kedalion Therapeutics, National Health and Medical Research Council, Rebecca L. Cooper Medical Foundation, Cornea and Contact Lens Society of Australia; Consultant, unrelated to this researche Alcon; Lecturere British Contact Lens Association; Patente Australian Provisional Patent Application 2023901150) submitted relating to the imaging method described in this article (IP owned by institution); Leadership orfiduciary role, not directly related to this researche Tear Film and Ocular Surface Society; Non-financial support, unrelated to this researche Medmont International.Supported in part by the Australian Research Council (ARC) Discovery Project (grant no.: DP230102105). The sponsor or funding organization had no role in the design or conduct of this research.